Hrvatin, S. et al. Neurons that regulate mouse torpor. Nature 583, 115–121 (2020).
Takahashi, T. M. et al. A discrete neuronal circuit induces a hibernation-like state in rodents. Nature 583, 109–114 (2020).
Zhang, Z. et al. Estrogen-sensitive medial preoptic area neurons coordinate torpor in mice. Nat. Commun. 11, 6378 (2020).
Upton, B. A., D’Souza, S. P. & Lang, R. A. QPLOT neurons—converging on a thermoregulatory preoptic neuronal population. Front. Neurosci. 15, 665762 (2021).
Yu, S. et al. Glutamatergic preoptic area neurons that express leptin receptors drive temperature-dependent body weight homeostasis. J. Neurosci. 36, 5034–5046 (2016).
Abbott, S. B. G. & Saper, C. B. Median preoptic glutamatergic neurons promote thermoregulatory heat loss and water consumption in mice. J. Physiol. 595, 6569–6583 (2017).
Tan, C. L. et al. Warm-sensitive neurons that control body temperature. Cell 167, 47–59.e15 (2016).
Lazarus, M. et al. EP3 prostaglandin receptors in the median preoptic nucleus are critical for fever responses. Nat. Neurosci. 10, 1131–1133 (2007).
Song, K. et al. The TRPM2 channel is a hypothalamic heat sensor that limits fever and can drive hypothermia. Science 353, 1393–1398 (2016).
Saper, C. B. & Machado, N. L. S. Flipping the switch on the body’s thermoregulatory system. Nature 583, 34–35 (2020).
Machado, N. L. S., Bandaru, S. S., Abbott, S. B. G. & Saper, C. B. EP3R-expressing glutamatergic preoptic neurons mediate inflammatory fever. J. Neurosci. 40, 2573–2588 (2020).
Vasilache, A. M., Andersson, J. & Nilsberth, C. Expression of PGE2 EP3 receptor subtypes in the mouse preoptic region. Neurosci. Lett. 423, 179–183 (2007).
Machado, N. L. S. et al. A glutamatergic hypothalamomedullary circuit mediates thermogenesis, but not heat conservation, during stress-induced hyperthermia. Curr. Biol. 28, 2291–2301.e2295 (2018).
Jendryka, M. et al. Pharmacokinetic and pharmacodynamic actions of clozapine-N-oxide, clozapine, and compound 21 in DREADD-based chemogenetics in mice. Sci. Rep. 9, 4522 (2019).
Nagai, Y. et al. Deschloroclozapine, a potent and selective chemogenetic actuator enables rapid neuronal and behavioral modulations in mice and monkeys. Nat. Neurosci. 23, 1157–1167 (2020).
Steiner, A. A., Antunes-Rodrigues, J. & Branco, L. G. Role of preoptic second messenger systems (cAMP and cGMP) in the febrile response. Brain Res. 944, 135–145 (2002).
Moffitt, J. R. et al. Molecular, spatial, and functional single-cell profiling of the hypothalamic preoptic region. Science 362, eaau5324 (2018).
Zhang, K. X. et al. Violet-light suppression of thermogenesis by opsin 5 hypothalamic neurons. Nature 585, 420–425 (2020).
Wechselberger, M., Wright, C. L., Bishop, G. A. & Boulant, J. A. Ionic channels and conductance-based models for hypothalamic neuronal thermosensitivity. Am. J. Physiol. Regul. Integr. Comp. Physiol. 291, R518–R529 (2006).
Nakamura, Y. et al. Prostaglandin EP3 receptor-expressing preoptic neurons bidirectionally control body temperature via tonic GABAergic signaling. Sci. Adv. 8, eadd5463 (2022).
Osterhout, J. A. et al. A preoptic neuronal population controls fever and appetite during sickness. Nature 606, 937–944 (2022).
Elmquist, J. K., Scammell, T. E., Jacobson, C. D. & Saper, C. B. Distribution of Fos-like immunoreactivity in the rat brain following intravenous lipopolysaccharide administration. J. Comp. Neurol. 371, 85–103 (1996).
Zhang, Z. et al. Primate preoptic neurons drive hypothermia and cold defense. Innovation 4, 100358 (2023).
Devic, P. et al. Late-onset post-lesional paroxysmal hypothermia: a case series and literature review. J. Neurol. 267, 3301–3309 (2020).
Zhang, S. et al. Hypothermia evoked by stimulation of medial preoptic nucleus protects the brain in a mouse model of ischaemia. Nat. Commun. 13, 6890 (2022).
Todd, W. D. et al. A hypothalamic circuit for the circadian control of aggression. Nat. Neurosci. 21, 717–724 (2018).
Vong, L. et al. Leptin action on GABAergic neurons prevents obesity and reduces inhibitory tone to POMC neurons. Neuron 71, 142–154 (2011).
Zhang, S. X. et al. Hypothalamic dopamine neurons motivate mating through persistent cAMP signalling. Nature 597, 245–249 (2021).
Mukamel, E. A., Nimmerjahn, A. & Schnitzer, M. J. Automated analysis of cellular signals from large-scale calcium imaging data. Neuron 63, 747–760 (2009).
Machado, N. L. S. & Saper, C. B. Genetic identification of preoptic neurons that regulate body temperature in mice. Temperature 9, 14–22 (2022).
Guillery, R. W. On counting and counting errors. J. Comp. Neurol. 447, 1–7 (2002).