Guo, B., Dong, R., Liang, Y. & Li, M. Haemostatic materials for wound healing applications. Nat. Rev. Chem. 5, 773–791 (2021).
Weisel, J. W. Enigmas of blood clot elasticity. Science 320, 456–457 (2008).
Jiang, S., Liu, S., Lau, S. & Li, J. Hemostatic biomaterials to halt non-compressible hemorrhage. J. Mater. Chem. B 10, 7239–7259 (2022).
Bao, G. et al. Liquid-infused microstructured bioadhesives halt non-compressible hemorrhage. Nat. Commun. 13, 5035 (2022).
Yuk, H. et al. Rapid and coagulation-independent haemostatic sealing by a paste inspired by barnacle glue. Nat. Biomed. Eng. 5, 1131–1142 (2021).
Ovsianikov, A., Khademhosseini, A. & Mironov, V. The synergy of scaffold-based and scaffold-free tissue engineering strategies. Trends Biotechnol. 36, 348–357 (2018).
Lancaster, M. A. & Knoblich, J. A. Organogenesis in a dish: modeling development and disease using organoid technologies. Science 345, 1247125 (2014).
Brandenberg, N. et al. High-throughput automated organoid culture via stem-cell aggregation in microcavity arrays. Nat. Biomed. Eng. 4, 863–874 (2020).
Stevens, A. J. et al. Programming multicellular assembly with synthetic cell adhesion molecules. Nature 614, 144–152 (2023).
Burdis, R. & Kelly, D. J. Biofabrication and bioprinting using cellular aggregates, microtissues and organoids for the engineering of musculoskeletal tissues. Acta Biomater. 126, 1–14 (2021).
Weisel, J. W. & Litvinov, R. I. Red blood cells: the forgotten player in hemostasis and thrombosis. J. Thromb. Haemost. 17, 271–282 (2019).
Garyfallogiannis, K. et al. Fracture toughness of fibrin gels as a function of protein volume fraction: mechanical origins. Acta Biomater. 159, 49–62 (2023).
Tutwiler, V. et al. Rupture of blood clots: mechanics and pathophysiology. Sci. Adv. 6, eabc0496 (2020).
Chan, K. Y. T. et al. The adhesion of clots in wounds contributes to hemostasis and can be enhanced by coagulation factor XIII. Sci. Rep. 10, 20116 (2020).
Hickman, D. A., Pawlowski, C. L., Sekhon, U. D. S., Marks, J. & Gupta, A. S. Biomaterials and advanced technologies for hemostatic management of bleeding. Adv. Mater. 30, 1700859 (2018).
Klein, M. K. et al. Development of optimized tissue-factor-targeted peptide amphiphile nanofibers to slow noncompressible torso hemorrhage. ACS Nano 14, 6649–6662 (2020).
Schell, H. et al. The haematoma and its role in bone healing. J. Exp. Orthop. 4, 5 (2017).
Shaikh, M. S., Zafar, M. S., Pisani, F., Lone, M. A. & Malik, Y. R. Critical features of periodontal flaps with regard to blood clot stability: a review. J. Oral Biosci. 63, 111–119 (2021).
da Silva Santos, S. C. N., Sigurjonsson, Ó. E., de Almeida Custódio, C. & da Luz Mano, J. F. C. Blood plasma derivatives for tissue engineering and regenerative medicine therapies. Tissue Eng. Part B Rev. 24, 454–462 (2018).
Gong, J. P., Katsuyama, Y., Kurokawa, T. & Osada, Y. Double-network hydrogels with extremely high mechanical strength. Adv. Mater. 15, 1155–1158 (2003).
Sun, J.-Y. et al. Highly stretchable and tough hydrogels. Nature 489, 133–136 (2012).
Zhao, X. et al. Soft materials by design: unconventional polymer networks give extreme properties. Chem. Rev. 121, 4309–4372 (2021).
Liu, W., Carlisle, C. R., Sparks, E. A. & Guthold, M. The mechanical properties of single fibrin fibers. J. Thromb. Haemost. 8, 1030–1036 (2010).
Lam, W. A. et al. Mechanics and contraction dynamics of single platelets and implications for clot stiffening. Nat. Mater. 10, 61–66 (2011).
Malette, W. G., Quigley, H. J., Gaines, R. D., Johnson, N. D. & Rainer, W. G. Chitosan: a new hemostatic. Ann. Thorac. Surg. 36, 55–58 (1983).
Dowling, M. B. et al. A self-assembling hydrophobically modified chitosan capable of reversible hemostatic action. Biomaterials 32, 3351–3357 (2011).
Kim, K. et al. Coagulopathy-independent, bioinspired hemostatic materials: a full research story from preclinical models to a human clinical trial. Sci. Adv. 7, eabc9992 (2021).
Jin, Z. et al. Gluing blood into gel by electrostatic interaction using a water-soluble polymer as an embolic agent. Proc. Natl. Acad. Sci. USA 119, e2206685119 (2022).
Lee, K. Y., Kong, H. J., Larson, R. G. & Mooney, D. J. Hydrogel formation via cell crosslinking. Adv. Mater. 15, 1828–1832 (2003).
Oliveira, B. L., Guo, Z. & Bernardes, G. J. L. Inverse electron demand Diels-Alder reactions in chemical biology. Chem. Soc. Rev. 46, 4895–4950 (2017).
Darko, A. et al. Conformationally strained trans-cyclooctene with improved stability and excellent reactivity in tetrazine ligation. Chem. Sci. 5, 3770–3776 (2014).
Tomarchio, E. G. et al. Tetrazine–trans-cyclooctene ligation: unveiling the chemistry and applications within the human body. Bioorg. Chem. 150, 107573 (2024).
Koo, H. et al. Bioorthogonal click chemistry-based synthetic cell glue. Small 11, 6458–6466 (2015).
Pol, M. et al. Dynamic modulation of matrix adhesiveness induces epithelial-to-mesenchymal transition in prostate cancer cells in 3D. Biomaterials 299, 122180 (2023).
Oneto, J. M. M., Khan, I., Seebald, L. & Royzen, M. In vivo bioorthogonal chemistry enables local hydrogel and systemic pro-drug to treat soft tissue sarcoma. ACS Cent. Sci. 2, 476–482 (2016).
Lux, S. E. IV. Anatomy of the red cell membrane skeleton: unanswered questions. Blood 127, 187–199 (2016).
Hoffman, J. F. Biconcave shape of human red-blood-cell ghosts relies on density differences between the rim and dimple of the ghost’s plasma membrane. Proc. Natl. Acad. Sci. USA 113, 14847–14851 (2016).
Jan, K.-M. & Chien, S. Role of surface electric charge in red blood cell interactions. J. Gen. Physiol. 61, 638–654 (1973).
Liu, X., Inda, M. E., Lai, Y., Lu, T. K. & Zhao, X. Engineered living hydrogels. Adv. Mater. 34, 2201326 (2022).
Nguyen, P. Q., Courchesne, N.-M. D., Duraj-Thatte, A., Praveschotinunt, P. & Joshi, N. S. Engineered living materials: prospects and challenges for using biological systems to direct the assembly of smart materials. Adv. Mater. 30, 1704847 (2018).
Liu, A. P. et al. The living interface between synthetic biology and biomaterial design. Nat. Mater. 21, 390–397 (2022).
Ducrot, E., Chen, Y., Bulters, M., Sijbesma, R. P. & Creton, C. Toughening elastomers with sacrificial bonds and watching them break. Science 344, 186–189 (2014).
Zhan, H., de Jong, H. & Löwik, D. W. P. M. Comparison of bioorthogonally cross-linked hydrogels for in situ cell encapsulation. ACS Appl. Bio Mater. 2, 2862–2871 (2019).
Shazeeb, M. S. et al. Assessment of in vivo degradation profiles of hyaluronic acid hydrogels using temporal evolution of chemical exchange saturation transfer (CEST) MRI. Biomaterials 178, 326–338 (2018).
Mikutis, S. & Bernardes, G. J. L. SnapShot: clinical and preclinical utility of click chemistry. Cell 186, 662–662.e1 (2023).
Sun, X. et al. Surface-engineering of red blood cells as artificial antigen presenting cells promising for cancer immunotherapy. Small 13, 1701864 (2017).
Maharjan, S. et al. Symbiotic photosynthetic oxygenation within 3D-bioprinted vascularized tissues. Matter 4, 217–240 (2021).
Ogden, R. W. & Roxburgh, D. G. A pseudo-elastic model for the Mullins effect in filled rubber. Proc. Math. Phys. Eng. Sci. 455, 2861–2877 (1999).
Yang, Z., Ma, Z., Liu, S. & Li, J. Tissue adhesion with tough hydrogels: experiments and modeling. Mech. Mater. 157, 103800 (2021).
Qi, Y., Caillard, J. & Long, R. Fracture toughness of soft materials with rate-independent hysteresis. J. Mech. Phys. Solids 118, 341–364 (2018).
Zhang, T., Lin, S., Yuk, H. & Zhao, X. Predicting fracture energies and crack-tip fields of soft tough materials. Extreme Mech. Lett. 4, 1–8 (2015).
Hui, C. Y., Ruina, A., Long, R. & Jagota, A. Cohesive zone models and fracture. J. Adhes. 87, 1–52 (2011).
Yuk, H. et al. Dry double-sided tape for adhesion of wet tissues and devices. Nature 575, 169–174 (2019).
Chen, X., Yuk, H., Wu, J., Nabzdyk, C. S. & Zhao, X. Instant tough bioadhesive with triggerable benign detachment. Proc. Natl Acad. Sci. USA 117, 15497–15503 (2020).
Nagahama, K., Kimura, Y. & Takemoto, A. Living functional hydrogels generated by bioorthogonal cross-linking reactions of azide-modified cells with alkyne-modified polymers. Nat. Commun. 9, 2195 (2018).
