St-Onge, M.-P. et al. Sleep duration and quality: impact on lifestyle behaviors and cardiometabolic health: a scientific statement from the American Heart Association. Circulation 134, e367âe386 (2016).
McAlpine, C. S. et al. Sleep modulates haematopoiesis and protects against atherosclerosis. Nature 566, 383â387 (2019).
Ziegler, K. A. et al. Immune-mediated denervation of the pineal gland underlies sleep disturbance in cardiac disease. Science 381, 285â290 (2023).
Laugsand, L. E., Vatten, L. J., Platou, C. & Janszky, I. Insomnia and the risk of acute myocardial infarction: a population study. Circulation 124, 2073â2081 (2011).
Daghlas, I. et al. Sleep duration and myocardial infarction. J. Am. Coll. Cardiol. 74, 1304â1314 (2019).
Clark, A., Lange, T., Hallqvist, J., Jennum, P. & Rod, N. H. Sleep impairment and prognosis of acute myocardial infarction: a prospective cohort study. Sleep 37, 851â858 (2014).
McAlpine, C. S. et al. Sleep exerts lasting effects on hematopoietic stem cell function and diversity. J. Exp. Med. 219, e20220081 (2022).
Hsueh, B. et al. Cardiogenic control of affective behavioural state. Nature 615, 292â299 (2023).
Critchley, H. D. & Harrison, N. A. Visceral influences on brain and behavior. Neuron 77, 624â638 (2013).
Mohanta, S. K. et al. Neuroimmune cardiovascular interfaces control atherosclerosis. Nature 605, 152â159 (2022).
Jin, H., Li, M., Jeong, E., Castro-Martinez, F. & Zuker, C. S. A bodyâbrain circuit that regulates body inflammatory responses. Nature 630, 695â703 (2024).
Wheeler, E. O. & White, P. D. Insomnia due to left ventricular heart failure unrecognized as such and inadequately treated. J. Am. Med. Assoc. 129, 1158â1159 (1945).
Madsen, M. T., Huang, C., Zangger, G., Zwisler, A. D. O. & Gögenur, I. Sleep disturbances in patients with coronary heart disease: a systematic review. J. Clin. Sleep Med. 15, 489 (2019).
Richards, D. A. et al. Distinct phenotypes induced by three degrees of transverse aortic constriction in mice. Sci. Rep. 9, 5844 (2019).
deAlmeida, A. C., van Oort, R. J. & Wehrens, X. H. T. Transverse aortic constriction in mice. J. Vis. Exp. https://doi.org/10.3791/1729 (2010).
Okamoto-Mizuno, K. & Mizuno, K. Effects of thermal environment on sleep and circadian rhythm. J. Physiol. Anthropol. 31, 14 (2012).
Swirski, F. K. & Nahrendorf, M. Leukocyte behavior in atherosclerosis, myocardial infarction, and heart failure. Science 339, 161â166 (2013).
Sager, H. B. et al. Targeting interleukin-1β reduces leukocyte production after acute myocardial infarction. Circulation 132, 1880â1890 (2015).
Liu, Z. et al. Fate mapping via Ms4a3-expression history traces monocyte-derived cells. Cell 178, 1509â1525.e19 (2019).
Cathomas, F. et al. Circulating myeloid-derived MMP8 in stress susceptibility and depression. Nature 626, 1108â1115 (2024).
Jacob, L. et al. Conserved meningeal lymphatic drainage circuits in mice and humans. J. Exp. Med. 219, e20220035 (2022).
Kirst, C. et al. Mapping the fine-scale organization and plasticity of the brain vasculature. Cell 180, 780â795.e25 (2020).
Cui, J., Xu, H. & Lehtinen, M. K. Macrophages on the margin: choroid plexus immune responses. Trends Neurosci. 44, 864â875 (2021).
Liddelow, S. A. Development of the choroid plexus and bloodâCSF barrier. Front. Neurosci. 9, 123479 (2015).
McAlpine, C. S. et al. Astrocytic interleukin-3 programs microglia and limits Alzheimerâs disease. Nature 595, 701â706 (2021).
Kiss, M. G. et al. Interleukin-3 coordinates glial-peripheral immune crosstalk to incite multiple sclerosis. Immunity 56, 1502â1514.e8 (2023).
Irwin, M. R. & Opp, M. R. Sleep health: reciprocal regulation of sleep and innate immunity. Neuropsychopharmacology 42, 129â155 (2017).
Rockstrom, M. D. et al. Tumor necrosis factor alpha in sleep regulation. Sleep Med. Rev. 40, 69â78 (2018).
Krueger, J. M. et al. Sleep as a fundamental property of neuronal assemblies. Nat. Rev. Neurosci. 9, 910â919 (2008).
Gent, T. C., Bandarabadi, M., Herrera, C. G. & Adamantidis, A. R. Thalamic dual control of sleep and wakefulness. Nat. Neurosci. 21, 974â984 (2018).
Sancho-Domingo, C., Carballo, J. L., Coloma-Carmona, A. & Buysse, D. J. Brief version of the Pittsburgh sleep quality index (B-PSQI) and measurement invariance across gender and age in a population-based sample. Psychol. Assess. 33, 111â121 (2021).
Dick, S. A. et al. Self-renewing resident cardiac macrophages limit adverse remodeling following myocardial infarction. Nat. Immunol. 20, 29â39 (2019).
Bajpai, G. et al. Tissue resident CCR2â and CCR2+ cardiac macrophages differentially orchestrate monocyte recruitment and fate specification following myocardial injury. Circ. Res. 124, 263â278 (2019).
Berntson, G. G. et al. Heart rate variability: origins, methods, and interpretive caveats. Psychophysiology 34, 623â648 (1997).
Manolis, A. A. et al. The role of the autonomic nervous system in cardiac arrhythmias: The neuro-cardiac axis, more foe than friend? Trends Cardiovasc. Med. 31, 290â302 (2021).
Carmeliet, P. & Tessier-Lavigne, M. Common mechanisms of nerve and blood vessel wiring. Nature 436, 193â200 (2005).
Gelosa, P. et al. Cerebral derailment after myocardial infarct: mechanisms and effects of the signaling from the ischemic heart to brain. J. Mol. Med. 100, 23â41 (2022).
Hoyer, F. F. et al. Tissue-specific macrophage responses to remote injury impact the outcome of subsequent local immune challenge. Immunity 51, 899â914.e7 (2019).
Thorp, E. B. et al. CCR2+ monocytes promote white matter injury and cognitive dysfunction after myocardial infarction. Brain. Behav. Immun. 119, 818â835 (2024).
Leistner, D. M. et al. Differential immunological signature at the culprit site distinguishes acute coronary syndrome with intact from acute coronary syndrome with ruptured fibrous cap: results from the prospective translational OPTICO-ACS study. Eur. Heart J. 41, 3549â3560 (2020).
Gerhardt, T. et al. Culprit plaque morphology determines inflammatory risk and clinical outcomes in acute coronary syndrome. Eur. Heart J. 44, 3911â3925 (2023).
Buysse, D. J., Reynolds, C. F., Monk, T. H., Berman, S. R. & Kupfer, D. J. The Pittsburgh sleep quality index: a new instrument for psychiatric practice and research. Psychiatry Res. 28, 193â213 (1989).
Horne, J. A. & Ostberg, O. A self assessment questionnaire to determine morningness eveningness in human circadian rhythms. Int. J. Chronobiol. 4, 97â110 (1976).
Full, K. M. et al. Validation of a physical activity accelerometer device worn on the hip and wrist against polysomnography. Sleep Health 4, 209â216 (2018).
Doench, J. G. et al. Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat. Biotechnol. 34, 184â191 (2016).
Sanson, K. R. et al. Optimized libraries for CRISPRâCas9 genetic screens with multiple modalities. Nat. Commun. 9, 5416 (2018).
Anzai, A. et al. The infarcted myocardium solicits GM-CSF for the detrimental oversupply of inflammatory leukocytes. J. Exp. Med. 214, 3293â3310 (2017).
Hilgendorf, I. et al. Ly-6chigh monocytes depend on nr4a1 to balance both inflammatory and reparative phases in the infarcted myocardium. Circ. Res. 114, 1611â1622 (2014).
Maki, K. A. et al. Sleep fragmentation increases blood pressure and is associated with alterations in the gut microbiome and fecal metabolome in rats. Physiol. Genomics 52, 280â292 (2020).
Topchiy, I., Fink, A. M., Maki, K. A. & Calik, M. W. Validation of PiezoSleep scoring against EEG/EMG sleep scoring in rats. Nat. Sci. Sleep 14, 1877â1886 (2022).
Yoo, J., Chepurko, V., Hajjar, R. J. & Jeong, D. Conventional method of transverse aortic constriction in mice. Methods Mol. Biol. 1816, 183â193 (2018).
Grune, J. et al. Neutrophils incite and macrophages avert electrical storm after myocardial infarction. Nat. Cardiovasc. Res. 1, 649â664 (2022).
Li, B. et al. Cumulus provides cloud-based data analysis for large-scale single-cell and single-nucleus RNA-seq. Nat. Methods 17, 793â798 (2020).
Wolf, F. A., Angerer, P. & Theis, F. J. SCANPY: large-scale single-cell gene expression data analysis. Genome Biol. 19, 15 (2018).
Korsunsky, I. et al. Fast, sensitive and accurate integration of single-cell data with Harmony. Nat. Methods 16, 1289â1296 (2019).
Aran, D. et al. Reference-based analysis of lung single-cell sequencing reveals a transitional profibrotic macrophage. Nat. Immunol. 20, 163â172 (2019).
Fang, Z., Liu, X. & Peltz, G. GSEApy: a comprehensive package for performing gene set enrichment analysis in Python. Bioinformatics 39, btac757 (2023).